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FRESH Bioprinting of Soft Biomaterials

In extrusion-based 3D bioprinting, working with soft materials, like collagen, presents a complex challenge given their inability to retain a desired shape following extrusion. One solution is
free form reversible embedding of suspended hydrogels (FRESH), a method of biofabrication which provides a temporary support and prevents the collapse or deformation of a construct during
bioprinting. LifeSupport™, a commonly used gelatin- based support bath for FRESH bioprinting, offers the added advantage that it can be easily removed after printing. This study aims to optimize the FRESH bioprinting of several popular biomaterials using LifeSupport bath to provide BIO X users with established printing protocols.