Workflows

Cell-line development

Simultaneous culture optimization and upscaling.
1

Non-cultured cellsuspension

Cytena F.Sight
2

Early static culture

Accurate and fast sorting and dispensing of single cells of interest
3

Automated cherry- picking of usefull and viable clones

Opentrons OT-2 (cherry picking)
4

Gentle washing, medium refreshing and automated cell-staining

Cytena C.WASH Plus

Microbiome research

Extreme time-saving and improved reliability.
1

Non-cultured suspension e.g. patient material

Cytena B.Sight
2

Accurate and fast sorting and dispensing of single cell: agar or liquid medium

3-5 days culture
3

NGS library prep on single-cell colonies

Opentrons Flex
4

Start sequencing

Monoclonal antibody production

1

Immunization of animal

2

Isolation of B-cells

Thinkcyte VisionSort: Ghost Cytometry
3

Single B-Cell Dispensing in lysisbuffer

Cytena F.Sight Omics
4

cDNA production and amplification

Opentrons Flex
5

Plasmid ligation & amplicon insertion

Opentrons Flex
6

Start of culture

Cytena C.Nest - Microbioreactor

Immunoassays

Improved accuracy and sensitivity.
1

Plate coating

Opentrons Flex
2

Non-contact washing and liquid dispensing

Cytena C.Wash Microplate washer and dispenser
3

Sample dilutions, addition and incubation

Opentrons Flex
4

Non-contact washing and e.g. blockbuffer dispensing

Cytena C.Wash
5

Repeat for subsequent washing and additions

Synthetic Biology

Seamless automation
1

Single cell sorting. Gently dispensed directly into PCR plate

Cytena F.Sight Omics
2

Single-cell Lysis

Low dispense volume: no dilution of lysisbuffer
3

mRNA processing and phage display

Opentrons Flex
4

Sorting of cells based on fluorescence

Cytena B.sight
5

Start early culture. Protein purification and analysis

With the Opentrons Flex
6

Start upscaling of selected cultures

Tecnic ePILOT bioreactor

Single-cell Omics

Gentle cell treatment, accuracy and versatility.
1

Single-cell dispensing with AOC technology: directly into PCR plate

Cytena F.Sight Omics
2

Single-cell Lysis

Single cells accurately dispensed in small volume lysisbuffer
3

NGS library prep / mRNA processing / Single-cell protein extraction

Opentrons Flex
4

Sequencing

5

Proteomics