S.NEST 2.0

Advanced culture platform combining incubation, dynamic mixing, and real-time DO/pH monitoring for optimized bioprocess and cell culture control.
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Overview

Effective Mixing at 96/24-Well Scale

Accelerate CLD workflows by 29–42%

By introducing true dynamic mixing culture and real-time DO/pH metabolic insight at the microplate stage, the platform enables faster, more reliable decision-making while maintaining clone integrity from the very beginning.

  1. Adjustable Mixing for Seamless Workflow Integration

    • Patented pneumatic mixing technology delivering precise, programmable mixing
    • Higher oxygen transfer rates and homogeneous medium distribution to support improved cell growth
    • Gentle, adjustable mixing enables true beneficial mixing even at the 96-well format
    • Effective and reproducible mixing at the 24-well scale to achieve shaker-ready cell numbers

  2. Real-Time, High-Resolution DO and pH Monitoring

    • Continuous in-well DO and pH monitoring reveals gradual changes in cellular metabolism over time
    • Sampling-free, non-invasive insight reduces contamination risk
    • Enables data-informed decision-making at the smallest scale of clone selection

  3. Optimized In-Well Environment & Multi-Chamber Scalability

    • Independent temperature, CO2, and humidity control in each incubation chamber
    • High-throughput 24-well/96-well format with individual well control
    • Scalable architecture supporting up to 16-chamber configurations
    • Compact C.BIRD system connects to any chamber for mixing

 

Technical Specifications

The S.NEST®, C.NEST®, and C.BIRD™ systems form a modern bioprocessing platform designed to support cell line development from single-cell expansion through early-stage screening.

S.NEST 2.0C.NEST 2.0C.BIRD
Mixing rate (sec/cycle)5–50 (±5%)5–50 (±5%)15–50 (±2%)
Suspension boostYesYesN/A
Throughput96 × 4 / 24 × 496 × 4 / 24 × 496 × 3 / 24 × 3
Medium Volume (µL/well)1000–1600 / 150–2001000–1600 / 150–2001000–1600 / 150–200
Incubation chambers44N/A
Temperature Control (°C)(RT+10) to 40(RT+10) to 40N/A
CO₂ Control (%)1–201–20N/A
Humidity MonitoringYesYesN/A
UV light SterilizationYesYesN/A
Data OutputDO / pHN/AN/A
Automation CompatibilityYesYesN/A
WorkstationYesYesN/A

Downloads

Application note: tool with real-time dissolved oxygen and pH monitoring
Brochure S.NEST
Customize the mixing level and increase cell growth with the S.NEST’s unique fluid control system
Incubate up to four 24- or 96-well plates at once for maximum productivity and efficiency
Optical sensors at the bottom of the unit monitor pH and dissolved oxygen (DO) values through the entire cell culture process
Valuable insights of pH/DO data throughout the culture period enable high throughput cell clone selection
Reduce the amount of time required to process cells

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